LOCUS       40924_24678        3073 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIT88 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H.
  TITLE     In vitro analyses of the production and activity of secondary small 
            interfering RNAs in C. elegans
  JOURNAL   EMBO J. 26 (24), 5007-5019 (2007)
  PUBMED    18007599
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Tabara H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2007) Contact:Hiroaki Tabara Kyoto University, 
            Graduate School of Medicine; Yoshida-Konoe, Building E, Room 208, 
            Sakyo, Kyoto 606-8501, Japan Fax :81-75-753-9311
REFERENCE   3  (bases 1 to 3073)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3073)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2007"; volume: "26"; issue: "24"; pages: "5007-5019"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2007) Contact:Hiroaki Tabara Kyoto University, Graduate 
            School of Medicine"; volume: " Yoshida-Konoe, Building E, Room 208, 
            Sakyo, Kyoto 606-8501, Japan Fax "; pages: "81-75-753-931"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    18..39
                     /label=trans-splicing leader sequence SL1
                     /note="trans-splicing leader sequence SL1"
     misc_feature    38..59
                     /note="multiple cloning sites containing restriction sites
                     (Stu I, Pst I, Xho I and Xba I)"
     misc_feature    59..119
                     /label=synthetic poly(A)61 region
                     /note="synthetic poly(A)61 region"
     misc_feature    115..131
                     /note="restriction enzyme sites (Bsa I and Spe I) for 
                     linearization of the plasmid"
     terminator      146..193
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(393..981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1155..2012)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2013..2117)
                     /label=AmpR promoter
     rep_origin      complement(2451..2906)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3051..3067
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggt ttaattaccc aagtttgagg cctgcagctc gagtctagaa
       61 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag
      121 agaccactag tccgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      181 gaggggtttt ttgctgaaag gaggaactat atccgggctt cctcgctcac tgactcgctg
      241 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      301 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      361 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      481 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      541 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      601 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      661 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      721 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      781 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      841 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      901 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      961 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1021 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1081 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1141 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1201 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1261 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1321 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1381 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1441 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1501 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1561 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1621 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1681 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1741 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1801 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1861 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1921 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1981 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2041 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2101 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2161 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     2221 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2281 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2341 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata
     2401 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa
     2461 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     2521 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     2581 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     2641 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     2701 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     2761 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     2821 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     2881 gcttaatgcg ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg
     2941 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3001 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     3061 ggccagtgaa ttg
//
